Reporter

Part:BBa_K1467203:Design

Designed by: Cara Deal   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)


GoldenGate compatible Bax - cell death inducer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 161
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.

Codon-optimised for expression in dicotyledenous plants.


Source

Mus musculus

References